inverted microscope hoffman modulation contrast equipment  (Nikon)

Journal: Cells

Article Title: The LEDGF/p75 Integrase Binding Domain Interactome Contributes to the Survival, Clonogenicity, and Tumorsphere Formation of Docetaxel-Resistant Prostate Cancer Cells

doi: 10.3390/cells10102723

Figure Lengend Snippet: Knockdown of LEDGF/p75, JPO2, menin, or HRP2 impairs tumorsphere formation in PC3-DR and DU145-DR cells. siRNA-mediated knockdowns of LEDGF/p75 ( A ), JPO2 ( B ), menin ( C ), and HRP2 ( D ) were confirmed by immunoblotting in PC3-DR and DU145-DR cells transfected with specific siRNAs (KD) compared to cells transfected with SD control oligos. Representative images of tumorsphere formation assays showing a decrease in spheroid size in PC3-DR and DU145-DR cells after individual siRNA-mediated depletion of LEDGF/p75, JPO2, menin, or HRP2 compared to SD control spheres. Tumorspheres were visualized using phase contrast or Hoffman Modulation Contrast microscopy. Tumorsphere area was quantified (bottom graphs) from triplicate images per experiment using ImageJ software. Data are from at least 3 independent experiments and are represented as mean SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: Tumorspheres were grown for 4 days at 37 °C/5% CO 2 and visualized in an Olympus IX70 microscope equipped with Phase Contrast and Hoffman Modulation Contrast, and a SPOT imaging system.

Techniques: Western Blot, Transfection, Microscopy, Software